John Libbey Eurotext

Annales de Biologie Clinique

MENU

Tri par billes magnétiques Technique et exemple du tri des lymphocytes T régulateurs CD25 + chez le rat Volume 62, issue 1, Janvier - Février 2004

Figures

See all figures

Authors
U 517 Inserm, Faculté de médecine, 7, boulevard Jeanne d‘Arc, 21000 Dijon. f.ghiringhellicompaqnet.fr
  • Key words: magnetic microsphere, immunomagnetic separation, cellular separation  
  • Page(s) : 73-8
  • Published in: 2004

Magnetic separation is a recent technology using magnetism properties. This technology is aimed to purify cells, cell organelles and biologically active compounds such as nucleic acids and proteins. Many magnetic separation procedures have been developed to isolate cells and molecules. The purpose of this review is to give an other view of various methods and strategies which can be employed for selection of targets cells and molecules. We describe techniques used to positive and negative cells selections, we show that magnetic selection is also aimed to perform multiparameter selection or functional selection. Then, we underline emerging technology used to select transfected cells and macromolecules such as proteins, DNA and RNA.