John Libbey Eurotext

Annales de Biologie Clinique


Recommendations for cerebrospinal fluid examination in acute leukemia Volume 75, issue 5, Septembre-Octobre 2017

Jean-François Lesesve Pour le Groupe francophone d’hématologie cellulaire (GFHC) 4
1 Service d’hématologie biologique, Centre de biologie et pathologie Est, LBMMS, Hospices civils de Lyon, France
2 Laboratoire d’hématologie, AP-HP Robert Debré, Paris, France
3 Laboratoire d’hématologie, CHU Côte de Nacre, Caen, France
4 Service d’hématologie biologique, CHU de Nancy, Vandoeuvre-lès-Nancy, France
* Tirés à part
  • Key words: cerebrospinal fluid, leukemia, recommendations
  • DOI : 10.1684/abc.2017.1250
  • Page(s) : 503-12
  • Published in: 2017

Cytological identification of blasts in cerebrospinal fluid in acute leukemia, lymphoid or myeloid, in adult and child, at diagnosis or during follow up lead to the diagnosis of leukemic meningitidis. Suitable CNS therapy based on a defined “CNS status” following an international standardized classification, lead to decrease cerebrospinal relapses. Established in 1993, this classification allows to treat patients based on their CNS status. Based on the red blood cells count, nucleated cells count and presence of blasts, it requires a standard technical procedure that guarantees the comparability of results coming from different medical laboratory. To improve the quality of cerebrospinal fluid analysis, in acute leukemias, preanalytical guidelines (turn around time), analytical guidelines (cytocentrifugation, adding serum protein, speed and duration of cytocentrifugation) and postanalytical guidelines (duration of conservation) are set by the Groupe francophone d’hématologie cellulaire.