John Libbey Eurotext

Annales de Biologie Clinique

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Preanalytical step of urinary protein measurement: from urine sampling to preparation for analysis of the specimen Volume 76, issue 6, Novembre-Décembre 2018

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Authors
Elizabeth Caussé Pour le groupe de travail SFBC, SFNDT, SNP « Actualités sur les protéinuries » 5 *
1 Laboratoire de biochimie et de biologie moléculaire, CHU de Tours, France
2 Laboratoire Oriade-Noviale, Montalieu-Vercieu, France
3 Laboratoire de biochimie et hormonologie, CHU de Montpellier, France
4 Laboratoire de biochimie, Groupement hospitalier de l’Est francilien, Site de Meaux, France
5 Laboratoire de biochimie, CHU de Toulouse, France
* Tirés à part
  • Key words: urinary protein, preanalytical step
  • DOI : 10.1684/abc.2018.1387
  • Page(s) : 609-16
  • Published in: 2018

The preanalytical phase is a key step in urinary protein measurement. It is a complex step, which includes urine sampling, storage and transport to the laboratory and preparation for analysis of the specimen. It can lead to numerous errors, since urine sampling is made by the patient himself. According to different cases, the procedures for urine sampling are presented in appendix 1 to 4. The aim of the presented guidelines is to improve the preanalytical step. In addition to the sampling made by a well-informed patient, the laboratory has to optimize transport and sample preservation, according to the analytes. The 24-hour urine collection is divided into aliquots with commercially available systems. Urinary proteins are measured on samples stored without preservatives. When 24-hour urine collection is not possible, a mid-stream urine sample is the most appropriate sample for protein measurement.