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Comparative study of the tissue distribution of equimolar repeated doses of hydroxocobalamin and cobalt chloride in the rats Volume 76, issue 2, Mars-Avril 2018

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Authors
1 Laboratoire de toxicologie, Groupe hospitalier Lariboisière-Fernand Widal, Assistance Publique-Hôpitaux de Paris, AP-HP, Paris, France
2 Laboratoire de biochimie, Hôpital Universitaire Necker-Enfants malades, Assistance Publique-Hôpitaux de Paris, AP-HP, Paris, France
3 Unité de technologies chimiques et biologiques pour la santé (UTCBS), CNRS UMR8258 - U1022, Faculté de pharmacie Paris Descartes, Université Paris Descartes, Paris, France
4 Département d’anesthésie-réanimation, Samu de Paris, Hôpital Universitaire Necker-Enfants malades, Assistance Publique-Hôpitaux de Paris, AP-HP, Paris, France
5 EA7323 Evaluation of Therapeutics and pharmacology in perinatality and pediatrics, Hôpitaux Universitaires Cochin-Broca-Hôtel-Dieu, Site Tarnier, Université Paris Descartes, Paris, France
6 Université Paris Diderot, Paris, France
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High dose of the cobalt atom is toxic for mammals. Hydroxocobalamin is considered safe due to the inclusion of the cobalt atom into the heminic moiety. The tissue distribution of cobalt following repeated doses of either hydroxocobalamin or cobalt chloride was studied in Wistar rats. In both cases, cobalt was administered in equimolar doses daily for an overall period of three weeks. Three groups were designed. In the hydroxocobalamin treated group, ten rats received hydroxocobalamin 17.5 mg by intraperitoneal route daily. In the cobalt-treated group, ten rats received cobalt chloride 3 mg i.p. daily. In the control group, six rats received a daily injection of 0.35 mL isotonic sodium chloride i.p. Cobalt concentrations were measured by Inductively Coupled Plasma Atomic Emission. Ours results showed that in rats having received either hydroxocobalamin or cobalt chloride, the tissue concentrations of cobalt were greater than those in the control group. The present study documented that in naive rats, the repeated administration of high doses of cobalt as hydroxocobalamin leads to tissue concentrations of the atom of cobalt significantly lower than those induced by equimolar doses of cobalt administered as cobalt chloride (p <0.05). We conclude that hydroxocobalamin reduced the tissue distribution of the cobalt atom in comparison with cobalt chloride.