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Annales de Biologie Clinique

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Improvement of the performances of the messages for the identification of the lymphocyte population with the Beckman Coulter ® LH750 TM haematology analyser Volume 67, issue 1, janvier-février 2009

Authors
Département d’hématologie, Centre hospitalier Emile Muller, Mulhouse

We have compared the efficiency of the detection for lymphocyte abnormalities using the LH 750 TM analyzer according to standard CLSI recommendations (Clinical and Laboratory Standards Institute) and three new rules dedicated to abnormal lymphocyte recognition. These new rules are defined by combining quantitative and qualitative criteria. They improve the detection of lymphocytes with abnormal morphology with an efficiency of 97,7% as compared to 81% with the standard protocol and a sensitivity of 92,8% compared to 23% initially. Regarding the detection of large granular lymphocytes, the new rules demonstrate an efficiency of 97,6%, a specificity of 81,8% and a specificity of 98,2%. These new criterias, which can be easily implemented in the settings of the automatic counter improve the technical efficiency of the validation process with an expected decrease of the number of manual slide reviews.