John Libbey Eurotext




Comparison of five techniques to detect anti-Saccharomyces cerevisiae antibodies (ASCA) in serum for diagnosing Crohn’s disease
Annales de Biologie Clinique. Volume 65, Number 6, 601-8, Novembre-Décembre 2007, article original
Résumé Article gratuit
Author(s) : C Le Goff, JP Chapelle, L Lutteri
Summary : Objective: the anti-Saccharomyces cerevisiae antibodies (ASCA) are diagnostic markers found in Crohn’s disease patients. The aim of this study was to compare three Elisa (enzyme linked immunosorbent assay) kits with the indirect immunofluorescence (IFI) technique and an immunodot for ASCA detection. Materials and methods: we compared the results obtained using IFI (IgA and IgG) and Elisa (IgA and IgG) in 139 patients (37 Crohn’s disease). An immunodot (IgA + IgG) was tested in a sub-group of 24 patients (18 Crohn’s disease). Results and discussion: for the different techniques by Elisa (IgA or IgG), the sensitivity ranged from 65% to 76%, the specificity from 88% to 98%, the positive predictive value (PPV) from 84% to 94% and the negative predictive value (NPV) from 88% to 93%. For IFI, the sensitivity was 81%, the specificity 100%, the PPV 100% and the NPV 93%. The immunodot showed a specificity and PPV of 100% and NPV of 33%. Conclusion: the detection of the ASCA is useful in the diagnosis of Crohn’s disease. IFI appears as the method of choice for its excellent sensitivity and specificity, and affordable costs.
Keywords : anti-Saccharomyces cerevisiae antibody, Crohn’s disease, Elisa, indirect immunofluorescence, immunodot

Comparison of five techniques to detect anti-Saccharomyces cerevisiae antibodies (ASCA) in serum for diagnosing Crohn’s disease