John Libbey Eurotext




Activity and stability of immobilized lipases in lipase-catalyzed modification of peanut oil
Oléagineux, Corps Gras, Lipides. Volume 11, Number 6, 464-8, NOVEMBRE-DÉCEMBRE 2004, Fondamental
Free Article
Author(s) : Mohamed M Soumanou, Aleodjrodo P Edorh, Uwe T Bornscheuer
Summary : Fatty acid release during lipolysis of peanut oil using microbial free and immobilized lipases in aqueous media was developed. Immobilized lipase from Rhizomucor miehei (RML) gave the best result from its ability to clive different fatty acids from peanut oil in such media. In organic solvent, interesterification of peanut oil with tricaprylin using immobilized lipases from RML, Chromobacterium viscosum (CVL) and Candida rugosa (CRL) was performed. The best substrate molar ratio of tricaprylin to peanut oil found was in the range 0.7 to 0.8. Using substrate molar ratio 0.7, high amount of structured triglyceride ST (about 35% MLM, 44% LML triglyceride fractions) was obtained with lipase from RML in n-hexane. The results found in solvent free system were in some cases quite similar to that obtained in organic solvent. In nine successive batch interesterification in solvent free medium using immobilized RML and CRL, no significant loss of amount of both produced triacylglycerol fractions until batch 7 was observed with RML.
Keywords : interesterification reaction, peanut oil, microbial lipases, immobilization, structured triacylglycerols, stability

Activity and stability of immobilized lipases in lipase-catalyzed modification of peanut oil