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Effects of magnesium sulfate on Na +,K + ‐ATPase and intracranial pressure level after cerebral ischemia |
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Magnesium Research. Volume 17, Number 3, 169-75, September 2004, ORIGINAL ARTICLE |
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 Free Article
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Author(s) : Mehmet Erkan Ustun, Hulagu Bariskaner, Alper Yosunkaya, Mehmet Gurbilek, Necdet Dogan |
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Summary : In the present study, the effects of magnesium sulfate on Na +,K +‐ATPase levels and intracranial pressure (ICP) after cerebral ischemia in rabbits were studied. Thirty New Zealand rabbits were divided into three groups. Group 1 was the control group. In group 2 (untreated group) cerebral ischemia was produced by clamping bilateral common carotid arteries for 60 min but in group 3 magnesium sulfate was administered 100 mg\\kg i.v. 10 min after opening the clamps. In group 1, ICP recordings were obtained 5, 60 and 120 min after craniectomy. In groups 2 and 3, ICP recordings were obtained 5 min after craniectomy but before clamping, 60 min after clamping and 60 min after opening the clamps. After taking ICP recordings, brain cortices were resected and Na +,K +‐ATPase activity was determined by subtracting the enzyme activity in the presence of ouabain from the total activity in the absence of ouabain method. There was a significant difference between Na +,K +‐ATPase levels of group 1 and group 2 ( P <\; 0.05). There was no significant difference in Na +,K +‐ATPase levels between group 1 and 3 ( P > 0.05), also preischemic ICP values were same in all groups ( P > 0.05). Preischemic and postischemic ICP values were significantly different between groups 1 and 2 ( P <\; 0.05), also postischemic (120 min) ICP values were significantly different between group 2 and group 3 ( P <\; 0.05). ICP values correlate well with Na +,K +‐ATPase level. These results demonstrate that cerebral ischemia leads to a decrease of ATPase level in the brain and magnesium sulfate suppresses the decrease of Na +,K +‐ATPase, also magnesium sulfate treatment improves the ICP changes. |
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Keywords : magnesium sulfate, cerebral ischemia, Na +‐K +‐ATPase, rabbit |
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