John Libbey Eurotext




Increased cell surface expression of histocompatibility class II IAd in c-FOS transfected clones of the P388D1 murine macrophage cell line.
European Cytokine Network. Volume 12, Number 1, 135-40, March 2001, Articles originaux
Free Article
Author(s) : Y. Le Garrec, E. Rouer, F. Conti, P. Michel, A. Pompidou
Summary : Numerous biological functions of the monocyte-macrophage lineage are affected by the presence of immunomodulators. Enhancement in transcription of c-fos has been shown in the murine P388D1 macrophage line treated by LPS, TPA, Ca++ ionophore or dibutyryl cAMP. In order to study the effects of an increased c-Fos protein level on macrophage functions, we previously have established stable c-fos-overexpressing clones in the P388D1 cell line. Here we report that the expression of class II MHC antigens (I-Ad antigen) is increased in these clones, particularly after IFN-g treatment. No variation in the cell surface expression of IFN-g receptor was observed. The increase of I-Ad cell surface expression was well correlated with the level of I-Ad mRNA. No inducible NO synthase activity and no increase of TNF-a release were observed in c-fos transfected cells. A slight increase of the basal expression of the main class II MHC transcription factor CIITA which is further amplified by IFN-g treatment, was observed in the c-Fos overexpressing clones. This suggests that the increased I-Ad expression in clones could result from a transactivating action of the c-Fos protein on the CIITA factor.
Keywords : MHC class II antigens, murine macrophages, c-Fos.

Increased cell surface expression of histocompatibility class II IAd in c-FOS transfected clones of the P388D1 murine macrophage cell line.