John Libbey Eurotext




Oncogenic activation of p21ras or pp60c-src in human colonic Caco-2 cells is associated with post-translational alterations of syndecan-1
Bulletin du Cancer. Volume 84, Number 3, 235-7, Mars 1997, Articles originaux
Résumé Article gratuit
Author(s) : Peggy Lévy, Annie Munier, Sophie Baron-Delage, Eric Chastre, Christian Gespach, Jacqueline Capeau, Gisèle Cherqui
Summary : The protein encoded by ras and src protooncogenes are frequently activated in a constitutive state in human colorectal cancer. In this study, we investigated the effect of oncogenic p21ras and Py-MT/pp60c-src on the synthesis of syndecan-1, a membrane anchored proteoglycan playing a role in cell-matrix interaction and neoplastic growth control. To this end, we used Caco-2 cells transfected with an activated (Val-12) human Ha-ras gene or the polyoma middle T (Py-MT) oncogene, a constitutive activator of pp60c-src tyrosine kinase activity. As compared to control vector-transfected Caco-2 cells, both oncogene-transfected cells exhibited: (1) a decrease in syndecan-1 specific activity; (2) a decrease in size and sulfation of syndecan-1 ectodomain glycosaminoglycan side chains; and (3) an active heparanase specifically degrading the heparan sulfate chains. In conclusion, the tumorigenic progression induced by oncogenic p21ras or Py-MT/pp60c-src is associated with marked alterations of syndecan-1 at the post-translational level.
Keywords : human colonic Caco-2 cells, ras, polyoma-middle T, pp60c-src, syndecan-1.

Oncogenic activation of p21ras or pp60c-src in human colonic Caco-2 cells is associated with post-translational alterations of syndecan-1